EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
2.4.2.8 | -999 |
- |
more |
increasing kcat with temperature (40-70°C) for all substrates tested |
685403 |
2.4.2.8 | -999 |
- |
more |
kcat of mutant enzymes |
638415 |
2.4.2.8 | -999 |
- |
more |
kcat/KM for 5-phospho-alpha-D-ribose 1-diphosphate = 0.00093 mM(-1)*min(-1) at 40°C, 0.00069 mM(-1)*min(-1) at 50°C, 0.00357 mM(-1)*min(-1) at 60°C, 0.00496 mM(-1)*min(-1) at 70°C |
685403 |
2.4.2.8 | -999 |
- |
more |
kcat/KM for guanine = 0.00262 mM(-1)*min(-1) at 50°C, 0.02631 mM(-1)*min(-1) at 70°C |
685403 |
2.4.2.8 | -999 |
- |
more |
kcat/KM for hypoxanthine = 0.00579 mM(-1)*min(-1) at 40°C, 0.00708 mM(-1)*min(-1) at 50°C, 0.01719 mM(-1)*min(-1) at 60°C, 0.03107 mM(-1)*min(-1) at 70°C |
685403 |
2.4.2.8 | -999 |
- |
more |
kcat/KM for hypoxanthine similar to kcat/KM for guanine, kcat/KM for xanthine approximately 20 times lower |
685403 |
2.4.2.8 | -999 |
- |
more |
kcat/KM for xanthine = 0.00040 mM(-1)*min(-1) at 50°C, 0.00139 mM(-1)*min(-1) at 70°C |
685403 |
2.4.2.8 | -999 |
- |
more |
kinetics |
638409 |
2.4.2.8 | -999 |
- |
more |
wild-type and diverse mutant enzymes, overview |
661233 |
2.4.2.8 | -999 |
- |
more |
wild-type and mutants: kcat for substrates hypoxanthine, guanine, xanthine, IMP, GMP, XMP, diphosphate, 5-phosphoribosyl 1-diphosphate |
638412 |