EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    6.3.2.3 | -78 |
- |
expression of the enzyme is absent after cryopreservation using increasing concentrations of DMSO at -78°C |
677199 |
| 6.3.2.3 | 0 |
30 |
purified recombinant enzyme, pH 7.8, 10 min, completely stable |
728286 |
| 6.3.2.3 | 37 |
- |
stable below |
1192 |
| 6.3.2.3 | 39.3 |
- |
mutant D24A enzyme,unfolding or transition midpoint (Tm) |
746387 |
| 6.3.2.3 | 41.3 |
- |
melting temperature, mutant V45W |
714064 |
| 6.3.2.3 | 42.5 |
- |
mutant R221A enzyme, unfolding or transition midpoint (Tm) |
746387 |
| 6.3.2.3 | 42.8 |
- |
melting temperature, mutant V44A/V45A |
714064 |
| 6.3.2.3 | 47.8 |
- |
melting temperature, mutant V45A |
714064 |
| 6.3.2.3 | 49.7 |
- |
mutant S42A enzyme, unfolding or transition midpoint (Tm) |
746387 |
| 6.3.2.3 | 50 |
- |
pH 8.3, 40% loss of activity after 1 h in 0.15 M Tris-HCl buffer |
1188 |
