EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
3.4.24.27 | -999 |
- |
thermostable neutral protease |
733899 |
3.4.24.27 | 0 |
- |
cold-adapted thermolysin-like protease, 20% activity remains |
734335 |
3.4.24.27 | 37 |
- |
incubation of impure enzyme at 37°C causes proteolysis of impurities, whereas the protease remains active and uncleaved |
695306 |
3.4.24.27 | 80 |
- |
1 h, heated enzyme has the same maximum velocity but lower affinity for substrate than the native |
31119 |
3.4.24.27 | 80 |
- |
first-order rate constant of the thermal inactivation at 80°C in the presence of 1-100mM CaCl2 |
734109 |
3.4.24.27 | 80 |
- |
half-life of wild-type enzyme is 18.3 min, half-lives of mutants are 25 min for L155A, 24 min for L155S, 60.8 min for L155A/I156V, 62.4 min for L155S/I156N, 93.3 for L155S/I156N, and 40 min for L155S/I156V |
717113 |
3.4.24.27 | 80 |
- |
the rate constant (kobs) for thermal inactivation at 80°C is 84000 s-1 for the wild type enzyme |
712532 |
3.4.24.27 | 80 |
- |
thermoinactivation of thermolysin at 80°C in 50% of solvents, half-lives of 3-20 min, with the increase in solvent hydrophobicity, thermal stability of the enzyme decreases, overview |
683691 |
3.4.24.27 | 82 |
- |
the enzyme loses 50% of its activity after 30 min at 82°C |
711724 |
3.4.24.27 | 85 |
- |
30 min, 51% remaining activity of recombinaqnt wild-type enzyme, 35-78% remaining activity of mutant enzymes |
683190 |