EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    2.7.4.9 | -999 |
- |
the denaturation temperature of the enzyme increase by 2.9°C in the presence of 5 mM ATP and by 8°C in the presence of 5 mM dTMP |
645201 |
| 2.7.4.9 | 40 |
- |
stable below |
737398 |
| 2.7.4.9 | 41 |
- |
10 min, inactivation when dTMP concentration is below 0.05 mM |
645196 |
| 2.7.4.9 | 42.5 |
- |
the midtemperature of thermal denaturation is 42.5°C |
706485 |
| 2.7.4.9 | 49 |
- |
Tm-value |
645201 |
| 2.7.4.9 | 50 |
- |
10 min, in absence of substrate activity decreases to 32% of the initial activity, completely stable in presence of 10 mM dTMP |
645191 |
| 2.7.4.9 | 57 |
- |
midpoint denaturation temperature for wild-type enzyme |
645202 |
| 2.7.4.9 | 57 |
- |
Tm-value |
645201 |
| 2.7.4.9 | 60 |
- |
10 min, 30% residual activity |
692915 |
| 2.7.4.9 | 60 |
- |
10 min, in absence of substrate activity decreases to 1% of the initial activity, partial protection in presence of 10 mM dTMP |
645191 |
