EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
1.14.14.1 | -999 |
- |
melting temperatures of the CYP102A1 monooxygenase and the CYP102A1 reductase domain differ by about 15°C: whereas Tm for the monooxygenase domain is 63°C, it is only 48°C for the reductase domain |
674972 |
1.14.14.1 | 27.5 |
- |
half-life of CYP102A7 at room temperature approximately 100 min. Increased activity (115%) after incubation at 27.5°C, higher temperatures result in a steady loss of activity against tetradecanoic acid. No activity after incubation at 60°C |
701835 |
1.14.14.1 | 46 |
- |
10 min, half-life, wild-type CYP102A1 |
674972 |
1.14.14.1 | 50 |
- |
in absence of NADPH, the enzyme retains about 85% of the CYP functional activity |
671729 |
1.14.14.1 | 50 |
67 |
the enzyme is moderately thermostable with an apparent melting temperature of 67°C and exhibits still 90% of initial activity after incubation at 50°C |
726753 |
1.14.14.1 | 61 |
- |
10 min, half-life, engineered CYP102A1 heme domain which utilizes H2O2 as electron donor |
674972 |