EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   1.14.13.8 | -999 |
- |
loss of about 30% of activity after heat treatment of mutant enzyme N413K, activity is preservable by NADPH |
703409 |
| 1.14.13.8 | -999 |
- |
the lung isozyme shows high thermal stability as the liver isozyme |
676315 |
| 1.14.13.8 | 15 |
45 |
15°C, 76% loss of activity, 45°C 99% loss of activity |
348501 |
| 1.14.13.8 | 24 |
- |
liver microsomes, enzyme inactivation after 20 days |
672958 |
| 1.14.13.8 | 35 |
- |
purified recombinant PTDH-mFMO, half-life is 5 h |
713168 |
| 1.14.13.8 | 37 |
- |
liver microsomes, enzyme inactivation after 48 h |
672958 |
| 1.14.13.8 | 38 |
- |
pH 7.6, half-life of free enzyme: 10 min, half-life of immobilized enzyme 5 h |
348486 |
| 1.14.13.8 | 40 |
- |
in absence of NADPH, residue 360 is important for thermal stability, half-lives of wild-type and mutant isozymes FMO1 and FMO3, overview |
673296 |
| 1.14.13.8 | 40 |
- |
purified recombinant FMO1, FMO3, and FMO5, the isozymes display similar thermal sensitivity, with activity decreased to 76% to 83% after 1 min preincubation at 40°C, 61% to 71% after 2 min preincubation, and 41% to 55% after 5 min preincubation |
687477 |
| 1.14.13.8 | 43.3 |
- |
enzyme mFMO falls into the category of moderately stable enzymes with an apparent melting temperature of 43.3°C |
764904 |
