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EC Number	Temperature Optimum [�C]	Temperature Optimum Maximum [�C]	Commentary	Reference
6.5.1.2	10	15	ligation of cohesive ends	1910
6.5.1.2	20	-	mutant enzyme L15F has 20fold lower ligation activity in vitro than wild-type enzyme	662787
6.5.1.2	24	37	cohesive-end DNA restriction fragments	1910, 1971
6.5.1.2	25	-	-	651212, 728580
6.5.1.2	27.7	-	p(dT)8, joining of thymidylate oligomers in the presence of poly(dA) as a template	1971
6.5.1.2	30	-	-	672811
6.5.1.2	34.5	-	p(dT)10, joining of thymidylate oligomers in the presence of poly(dA) as a template	1971
6.5.1.2	37	-	-	661248
6.5.1.2	37	-	p(dT)12-18, joining of thymidylate oligomers in the presence of poly(dA) as a template	1971
6.5.1.2	40	-	-	716944

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Release 2023.1 (January 2023)