EC Number |
---|
6.1.1.18 | - |
6.1.1.18 | DNA sequence determination and analysis, enzyme contains a N-terminal lysine-rich sequence KPKKKKEK, that may function as a nuclear targetng signal as well as in regulation of gene expression, since the 8 amino acid peptide derived from the motif interacts with DNA and changes the DNA molecule form from B to Z form |
6.1.1.18 | expressed in Escherichia coli BL21(DE3) cells |
6.1.1.18 | expressed in Saccharomyces cerevisiae strain BCY123 |
6.1.1.18 | expression of full length and C-terminal truncated GlnRS, lacking the the Yqey domain, and of the isolated Yqey protein, in Escherichia coli strain ER2566 |
6.1.1.18 | expression of His-tagged GlnRS mutants in strain BL21-DE3 |
6.1.1.18 | expression of the His-tagged chimeric mutant enzyme in Escherichia coli strain BL21(DE3) or the temperature sensitive strain JP1449(DE3) |
6.1.1.18 | gene GLN4, phylogenetic analysis |
6.1.1.18 | gene glnS, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 |
6.1.1.18 | gene QARS, genotyping, recombinant expression of codon-optimized His-tagged wild-type and mutant enzymes in Escherichia coli |