EC Number   |
|---|
   4.2.2.1 | - |
| 4.2.2.1 | 293 cells stably express Hyal-2 |
| 4.2.2.1 | cloning overexpression, and purification of SagHL and its different fragments/domains |
| 4.2.2.1 | cloning, overexpression and purification of the C-terminal domain swapped chimeras of SpnHL and SagHL (hyaluronate lyase from Streptococcus agalactiae), modified enzyme SpnHLv/SagHLv (where the size of linker connecting the alpha-domain to C-terminal domain is enlarged by two amino acid) |
| 4.2.2.1 | complementation of an enzyme-deficient mutant strain by expression of hysA |
| 4.2.2.1 | DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and truncation and point mutant enzymes in Escherichia coli strain BL21(DE3) using plasmid pSF49 |
| 4.2.2.1 | DNA sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli |
| 4.2.2.1 | expressed in Escherichia coli TOP10 |
| 4.2.2.1 | expression in Escherichia coli |
| 4.2.2.1 | expression in Escherichia coli BL21(DE3) |
