EC Number   |
|---|
    4.2.1.2 | - |
| 4.2.1.2 | a pfl ldhA double mutant Escherichia coli strain NZN11 is used to produce succinic acid by overexpressing the Escherichia coli malic enzyme gene sfcA. This strain, however, produces a large amount of malic acid as well as succinic acid. The fumB gene encoding the anaerobic fumarase of Escherichia coli is co-amplified to solve the problem of malic acid accumulation, and subsequently improve the succinic acid production |
| 4.2.1.2 | Cos-1 cells transfected with fumarase constructs, human fumarase with either the native or cytochrome c oxidase subunit VIII mitochondrial targeting sequence is detected exclusively in mitochondria in more than 98% of the cells, while the remainder 1-2% of the cells shows varying amounts of nuclear labeling. When human fumarase is fused to the yeast mitochondrial targeting sequence, more than 50% of the cells show nuclear labeling |
| 4.2.1.2 | entire protein-coding region of fumarase cDNA cloned from Dahl salt-sensitive, SS-13BN, and Brown Norway rats. PCR product inserted into the T-easy vector and propagated in competent Escherichia coli |
| 4.2.1.2 | expressed in Escherichia coli |
| 4.2.1.2 | expressed in Escherichia coli as a His-tagged fusion protein |
| 4.2.1.2 | expressed in Escherichia coli as His-tagged fusion proteins |
| 4.2.1.2 | expressed in Escherichia coli strain JM109 |
| 4.2.1.2 | expressed in HK-2 cells and in skin fibroblasts |
| 4.2.1.2 | expressed in yeast FUM1 mutant strain |
