EC Number   |
|---|
   4.1.99.13 | - |
| 4.1.99.13 | a cDNA of (6-4)photolyase from Dunaliella salina is cloned, sequenced and its amino acid sequence is deduced |
| 4.1.99.13 | cloned and overexpressed in Escherichia coli |
| 4.1.99.13 | DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis |
| 4.1.99.13 | due to insolubility problems (6-4) photolyases is overexpressed as a fusion protein in Escherichia coli. Plasmid pXZ1997, a derivative of pMal-c2 containing the Drosophila melanogaster phr(6-4) cDNA fused in frame to the malE gene encoding maltose-binding protein (MBP), is propagated in Escherichia coli strain UNC523 (phr::kan uvrA::Tn10) selecting for ampicillin resistance. Cells are cultured in 2 liter of LB to A600: 0.60.8. IPTG is added to 0.3 mM, and incubation continued for 6 h prior to harvesting the cells by centrifugation. |
| 4.1.99.13 | expressed as a GST-fusion protein in Escherichia coli |
| 4.1.99.13 | expressed as a GST-tagged fusion protein in Escherichia coli |
| 4.1.99.13 | expressed as a GSTtagged fusion protein in Escherichia coli |
| 4.1.99.13 | expressed as a His-tagged fusion protein |
| 4.1.99.13 | expressed in Escherichia coli |
