EC Number   |
|---|
   3.5.5.1 | - |
| 3.5.5.1 | a recombinant Escherichia coli BL21(DE3)/pET28a-cyc705 was constructed to achieve the heterologous expression of cyc705 |
| 3.5.5.1 | cloned into expression vector pET-28a and expressed in Escherichia coli strain BL21(DE3) |
| 3.5.5.1 | DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) under the control of T7 lac promoter using pET-28a to give pET-Nit, lactose is best as inducer in different media, method optimization |
| 3.5.5.1 | DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression in Escherichia coli strain BL21-Gold(DE3)/pOK101/pTf16, no cleavage of the C-terminal peptide of the enzyme in the recombinant bacteria |
| 3.5.5.1 | DNA and amino acid sequence determination, genes of the 3 isozymes are clustered on chromosome 3 |
| 3.5.5.1 | expressed in Escherichia coli |
| 3.5.5.1 | expressed in Escherichia coli BL21 CodonPlus (DE3) RIL cells |
| 3.5.5.1 | expressed in Escherichia coli BL21(DE3) cells |
| 3.5.5.1 | expressed in Escherichia coli DH5alpha cells |
