EC Number   |
|---|
    3.5.4.4 | - |
| 3.5.4.4 | enzyme expression in an enzyme-deficient Escherichia coli strain Sphi3834 |
| 3.5.4.4 | expressed in Escherichia coli BL21(DE3) |
| 3.5.4.4 | expression in Escherichia coli |
| 3.5.4.4 | expression in Escherichia coli BL21(DE3) |
| 3.5.4.4 | expression in Escherichia coli S Phi 3834 |
| 3.5.4.4 | expression of His-tagged enzyme in Escherichia coli strain BL21 |
| 3.5.4.4 | isozyme ADA2 is expressed in S2 Drosophila cells |
| 3.5.4.4 | overexpression in Escherichia coli BL21 (DE3) |
| 3.5.4.4 | the activity of human adenosine deaminase (hADA) expressed in transgenic seeds of three different plant species: pea (Pisum sativum L.), Nicotiana benthamiana L. and tarwi (Lupinus mutabilis Sweet) is compared. All three species are transformed with the same expression vector containing the hADA gene driven by the seed-specific promoter LegA2 with an apoplast targeting pinII signal peptide. During the study, several independent transgenic lines are generated and screened from each plant species and only lines with a single copy of the gene of interest are used for hADA expression analysis. A stable transgenic canola line expressing the ADA protein, under the control of 35S constitutive promoter is used as both as a positive control and for comparative study with the seed specific promoter. The highest activity of the hADA enzyme (U/g seed) is reported in tarwi (4.26 U/g) followed by pea (3.23 U/g) and Nicotiana benthamiana (1.69 U/g) |
