EC Number   |
|---|
   3.4.24.27 | an efficient method of production of enzymatically active thermolysin by refolding the protein expressed as inclusion bodies in Escherichia coli. Association of high pressure and alkaline pH is very efficient for solubilization |
| 3.4.24.27 | cloned in Bacillus subtilis DB117, plasmids subcloned in Escherichia coli |
| 3.4.24.27 | expressed in Escherichia coli strain K12 JM109 |
| 3.4.24.27 | expression of wild-type and mutant enzymes in Bacillus subtilis by vector pTB51, the enzymes are secreted to the culture medium |
| 3.4.24.27 | expression of wild-type and mutant enzymes in Escherichia coli K12 strain JM109 |
| 3.4.24.27 | expression of wild-type and mutant enzymes in Escherichia coli K12 strain JM109 and secretion to the cell culture medium |
| 3.4.24.27 | expression of wild-type and mutant enzymes in Escherichia coli strain T5KC991 |
| 3.4.24.27 | gene HSPA, DNA and amino acid sequence determination and analysis, sequence comparison |
| 3.4.24.27 | gene npr, DNA and amino acid sequence determination, expression of wild-type and mutant enzymes in Escherichia coli K12 strain JM109 |
| 3.4.24.27 | gene npr, expression of wild-type and mutant enzymes in Escherichia coli K12 strain JM109 |
