EC Number   |
|---|
   3.4.21.68 | - |
| 3.4.21.68 | a truncated version of tPA is fused to the N terminus of the phasin protein with a thrombin cleavage site as the linker, and then expressed in Escherichia coli strain XL1-Blue on the surface of polyhydroxybutyrate granules using phasin as the affinity tag. Untreate enzyme is expressed in inactive inclusion bodies in the bacteria. The in vivo surface display strategy for functional rPA expression in Escherichia coli is distinct for its efficient folding and easier purification, method optimization, overview. Subcloning in Escherichia coli strain DH5alpha |
| 3.4.21.68 | astrocytes co-transfected with metalloproteinase-9 or tPA luciferase reporter plasmid and pCMV-beta-galactosidase reporter plasmid |
| 3.4.21.68 | cDNA expressed in Escherichia coli in the form of inclusion body |
| 3.4.21.68 | construction of an oviduct-specific vector containing tissue plasminogen activator protein and green fluorescent protein (pL-2.8OVtPAGFP), and expression in vitro and in vivo in hen eggs, and in oviduct epithelial and 3T3 cells, compared to control vector pEGP-N1. The oviduct-specific vector pL-2.8OVtPAGFP is expressed only in oviduct epithelial cells, no detection in heart, muscle, liver and intestine, whereas pEGP-N1 is detected in oviduct epithelial and 3T3 cells. tPA expressed in egg white and oviduct epithelial cells shows fibrinolytic activity. Subcloning in Escherichia coli |
| 3.4.21.68 | construction of plastid transformation vector pKCZK2S for expressing the K2S gene in Nicotiana tabacum chloroplast genome under the control of the constitutive Prrn promoter, and the 3' UTR of the Chlamydomonas rbcl gene as a terminator, recombinant expression of His6-tagged truncated form of the tissue plasminogen activator, K2S, in Nicotiana tabacum leaf chloroplasts |
| 3.4.21.68 | evaluation of expression methods, overview. Recombinant expression of active full-length and active truncated 650 bp enzymes in Nicotiana tabacum |
| 3.4.21.68 | expressed in COS-1 cells |
| 3.4.21.68 | expressed in HeLa cells and MCF-7 cells |
| 3.4.21.68 | expressed in SF9 cells using the Bac-to-Bac baculovirus system |
