EC Number |
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3.4.21.108 | - |
3.4.21.108 | barely expressed full-length HtrA2/Omi as a GST fusion protein in Escherichia coli BL21 |
3.4.21.108 | coexpression of Flag-tagged and Ndelta133 HtrA2 proteins and a myc-epitope-tagged GRIM-19 protein in MCF-7 cells. MCF-7 cells transfected with expression vectors coding for GFP-HtrA2 and GFP-HtrA2-S306A and GRIM-19 |
3.4.21.108 | constructs encoding C-terminal His6-tagged full-length dOmi or truncated mutants are generated using the pET-21a and the pVL-1393 transfer vector, proteins are overexpressed in Escherichia coli BL21DE3 and Sf9 insect cells, respectively |
3.4.21.108 | expressed in Escherichia coli (BL21) transformed with pRSET containing cDNA for full-length wild-type Omi or mutant |
3.4.21.108 | expressed in human embryonic kindney cells and in Escherichia coli |
3.4.21.108 | expressed in yeast as a bait protein in a two-hybrid assay and by transient transfection of Human embryonic kidney (HEK)-293 cells |
3.4.21.108 | expression as GST fusions in Escherichia coli BL21 |
3.4.21.108 | expression from pET-15b |
3.4.21.108 | expression in Escherichia coli |