EC Number   |
|---|
    3.2.2.5 | - |
| 3.2.2.5 | cloning of inactive enzyme variant SPNH5 from strain HSC5 and of active variant SPNJ4, expression of C-terminally HA-tagged and His6-tagged variant SPNJ4 in Escherichia coli strain TOP10 |
| 3.2.2.5 | co-expression of N-terminally His6-tagged enzyme with inhibitor IFS in Escherichia coli strain Rosetta2 (DE3) |
| 3.2.2.5 | DNA and amino acid sequence determination and analysis, genomic structure, recombinant expression of FLAG-tagged wild-type and mutant enzymes in HEK293 cells |
| 3.2.2.5 | enzymatic domain of CD38 |
| 3.2.2.5 | expressed in Pichia pastoris |
| 3.2.2.5 | expression in Escherichia coli, FLAG-tag |
| 3.2.2.5 | expression in THP-1 cells, selection of transfected cells by G418 |
| 3.2.2.5 | expression of extracellular region of CD38 in Drosophila S2 cells |
| 3.2.2.5 | expression of extramembrane domain in Saccharomyces cerevisiae |
