EC Number   |
|---|
    3.2.1.8 | - |
| 3.2.1.8 | 3588-bp BamHI-XbaI fragment containing genes xyl30 and hd30 introduced into the pN702GEM3 plasmid and overexpressed in Streptomyces lividans strain 66 |
| 3.2.1.8 | Abf62A-Axe6A is a modular enzyme comprising (in order from the N-terminus): an N-terminal signal peptide, a glycoside hydrolase family 62 (GH62) catalytic module, a family 6 carbohydrate binding module (CBM6), a dockerin module and an additional carbohydrate esterase family 6 catalytic module (CE6). Three Abf62A-Axe6A derivatives are constructed, overexpressed in Escherichia coli, purified, and biochemically characterized: 1. RjAbf62A-Axe6A, containing all four modules but lacking the signal peptide, 2. RjAbf62A-CBM6, containing the GH62 and CBM6 modules, and 3. RjAxe6A, containing only CE6 |
| 3.2.1.8 | amplified fragment of Xyn11 (nucleotides 64-646) cloned into the pSE420-CHis vector and transformed into Escherichia coli strain XL1-Blue MR |
| 3.2.1.8 | cloning in Escherichia coli strain DH10B, overexpression of wild-type and mutant enzymes in Pichia pastoris strain X-33 |
| 3.2.1.8 | constitutive expression in Pichia pastoris |
| 3.2.1.8 | construction of endoglucanase GE40 (EC 3.2.1.4) from Geobacillus thermodenitrificans with a signal peptide sequence derived from the endoxylase encoding gene GtxynA1 from Geobacillus thermodenitrificans strain T12, overview. Recombinant expression of the enzyme in Escherichia coli strain BL21(DE3) (under control of the lac operator) and in Geobacillus thermodenitrificans strain T12 |
| 3.2.1.8 | CtXynGH30, recombinant overexpression in Escherichia coli strain BL21 |
| 3.2.1.8 | DNA and amino acid sequence determination and analysis, expression in Escherichia coli |
| 3.2.1.8 | DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 |
