EC Number   |
|---|
    3.2.1.21 | - |
| 3.2.1.21 | bgl operon, gene bglB, contruction of a genomic library, DNA and amino acid sequence determination and analysis, determination of bgl operon structure and organization, overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) as His-tagged proteins, subcloning in Escherichia coli strain DH5alpha |
| 3.2.1.21 | cDNA expressed in insect cells, Baculovirus expression system |
| 3.2.1.21 | cDNA is expressed in insect cells, Baculovirus expression system |
| 3.2.1.21 | cloned in frame with the Saccharomyces cerecisiae a-factor secretory signal and expressed in Pichia pastoris strain X-33 |
| 3.2.1.21 | cloned into the pET-28b vector. His-tagged MeBglD2 is expressed in Escherichia coli BL21(DE3) cells |
| 3.2.1.21 | DNA and amino acid sequence determination and anaylsis, expression analysis by semi-quantitative RT-PCR, stable expression of the 35S-GmICHG:GFP fusion gene in Arabidopsis thaliana T87 cells using an Agrobacterium tumefaciens GV3101(pMP90) transformation system, phylogenetic analysis |
| 3.2.1.21 | enzyme with an N-terminal truncation following the propeptide and eight histidine residues enabling its purification by immobilized metal-ion affnity chromatography, functional, methanol-inducible enzyme expression in Pichia pastoris strain GS115 and Saccharomyces cerevisiae strains DY150 and DBY746, addition of 0.5% casamino acids to the culture medium stabilizes the pH of the culture and increaseds the protein yield by 3-5folds, insertion of a polyhistidine-tag either after the N-terminal alpha-factor signal sequence or at the C-terminus fails to assist in purification by immobilized metal-ion affnity chromatography due to post-translational processing at both termini, expression in Escherichia coli strain BL21(DE3) as insoluble and inactive protein, which can be made soluble by co-expression with bacterial chaperonin GroESL |
| 3.2.1.21 | expressed in Aspergillus oryzae |
| 3.2.1.21 | expressed in Escherichia coli |
