EC Number   |
|---|
   3.1.26.13 | - |
| 3.1.26.13 | cloning a His-tagged p66 or p51 HIV-1 RT DNA fragment into the pQE-9 vector and expression in Escherichia coli strain BL21(DE3)pLys |
| 3.1.26.13 | expressed in Escherichia coli BL21(DE3) cells |
| 3.1.26.13 | expressed in Escherichia coli XL1 Blue cells |
| 3.1.26.13 | expression in Escherichia coli |
| 3.1.26.13 | expression of His-tagged enzyme |
| 3.1.26.13 | expression of His6-tagged p66/p51 HIV-1 reverse transcriptase p51/p66 52A variant C280S, the p66 subunit also contains the mutations K172A and K173A |
| 3.1.26.13 | expression of RNase H domain from residue Y427 to L560 as fusion protein in Escherichia coli |
| 3.1.26.13 | expression of RNase H, in an N-terminally His-tagged construct designed to encode the p51 subunit joined by a linker to the thumb (T), connection (C), and RNase H (R) domains of p66, in Escherichia coli strain MIC2067(DE3) lacking endogenous RNase HI and HII as soluble protein. The construct G provides sufficient RNase H activity to complement Escherichia coli growth at 42°C |
| 3.1.26.13 | expression of the isolated RNase H domain in Escherichia coli |
