EC Number |
---|
3.1.1.2 | - |
3.1.1.2 | a high level of recombinant hPON1 type A (rPON1A) is produced by Hi-5 insect cells. A fraction is secreted into the cell culture medium, but the majority remains associated with the host insect cells |
3.1.1.2 | application of directed evolution in achieving functional expression of PON1 and PON3 in Escherichia coli culture and a dramatic increase in their hydrolytic proficiency toward the fluorogenic organophosphate substrate 7-O-diethylphosphoryl-3-cyano-7-hydroxycoumarin. The powerful tool of directed evolution opens new prospects for improving their phosphotriesterase activity toward other hazardous organophosphates and toward catalytic activities related to the prevention of atherosclerosis |
3.1.1.2 | bacterially expressed chimeric G2E6 |
3.1.1.2 | directed evolution of phosphotriesterase from Pseudomonas diminuta for heterologous expression in Escherichia coli results in stabilization of the metal-free state |
3.1.1.2 | expressed from Trichoplusia ni larvae |
3.1.1.2 | expressed in baculovirus system in Hi5 insect cells |
3.1.1.2 | expressed in Drosophila melanogaster |
3.1.1.2 | expressed in Escherichia coli |
3.1.1.2 | expressed in Escherichia coli BL21(DE3) cells |