EC Number   |
|---|
    2.8.4.1 | - |
| 2.8.4.1 | expression of two ORF gene products in Escherichia coli DS 410, expressed proteins are suggested to be important for enzyme activity, but neither stimulatory nor inhibitory effects of these gene products are determined |
| 2.8.4.1 | gene mcr, sequencing and analysis of mcr-containing archaeal metagenome-assembled genomes (MAGs) from several hot springs, phylogenetic analysis |
| 2.8.4.1 | genes in operons mrt and mcr, DNA and amino acid sequence determination, promoter region, determination of operator regions of the mcr and mrt operons, and of cis elements and trans-acting factors responsible for the gene expression of MCRs by using electrophoretic mobility shift assay and affinity particle purification. IMP dehydrogenase-related protein VII, IMPDH VII encoded by MTH126, is a plausible candidate for the transcriptional regulator of the mcr operon in this methanogen, the binding site of IMPDH VII mostly overlaps the factor B-responsible element-TATA box of the mcr operon, overview. Expression in Escherichia coli strain BL21(DE3) |
| 2.8.4.1 | genetic organization of the mcr operon, overview |
| 2.8.4.1 | mcrBDCGA operon, genes Metok_0956, Metok_0960, and Metok_0957, recombinant expression of His-tagged MCRok from thermophile Methanothermococcus okinawensis (rMCRok) in mesophile Methanococcus maripaludis |
| 2.8.4.1 | metagenome-assembled genome, Mcr alpha, beta, and gamma genes, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, metabolic reconstruction of the Candidatus Polytropus marinifundus genome |
| 2.8.4.1 | recombinant enzyme constitutive expression in Arabidopsis thaliana under the control of CaMV35S promoter via an Agrobacterium-mediated floral-dip transformation, semi-quantitative RT-PCR and quantitative real-time PCR enzyme expression analysis |
| 2.8.4.1 | recombinant expression of Strep-tagged enzyme |
