EC Number   |
|---|
   2.7.12.2 | - |
| 2.7.12.2 | A549 cells transfected with wild-type or constitutively active MEK1 cDNA, both MEK1 expressions lead to increased SMAD3 expression and ERK phosphorylation |
| 2.7.12.2 | bacterial expression |
| 2.7.12.2 | cloning of cotton group B MAPKK gene GhMKK3, the gene has 6 introns, sequence comparisons and phylogenetic analysis, overexpression in Nicotiana benthamiana, RNA sequencing and quantitative real-time PCR analysis. fusion Transient expression of the GFP-tagged enzyme in Nicotiana benthamiana leaves using Agrobacterium infiltration (agroinfiltration) or transiently transformed into onion epidermal cells using particle bombardment |
| 2.7.12.2 | constitutive active form of MEK1 and siRNA-targeting MEK1 cloned into MSCV-IRES-GFP vector, retrovirally transduced to HSCs and injected into sublethally irradiated RAG2-/- (CD42.2) mice |
| 2.7.12.2 | construction of a 35S:MKK3-WT:myc expression cassette, either myc epitope-tagged wild-type MKK3 or constitutively active MKK3 are transiently expressed in Arabidopsis thaliana protoplasts, co-overexpression of MKK3 with MKK7 in ProPR1:GUS transgenic cell line, interaction analysis of MKK3 with group C MAPK kinases by yeast two-hybrid system, MKK3 and MPK7 induce the PR1 promoter, overview |
| 2.7.12.2 | DMKK4, DNA and amino acid sequence determination |
| 2.7.12.2 | DNA and amino acid sequence determination and analysis, expression of wild-type and mutant GST-fusion enzymes in Escherichia coli, expression of a GFP-tagged enzyme in the null mutant strain, complementation of the null mutant strain by expression of the enzyme from plasmid or by reintegration of the wild-type gene into the genomic DNA locus |
| 2.7.12.2 | expressed in COS7 cells |
| 2.7.12.2 | expression in COS and 293 cells |
