EC Number   |
|---|
    2.5.1.78 | - |
| 2.5.1.78 | construction of a fusion protein with enzyme BLS and inner core domain (VP8d) of VP8 spike protein from bovine rotavirus, subcloning in Escherichia coli, expression of the BLS-VP8d fusion protein in Nicotiana tabacum cv. Petit Havana leaf chloroplasts to evaluate the possible use of this antigen for vaccine development |
| 2.5.1.78 | expression in Escherichia coli |
| 2.5.1.78 | expression in Escherichia coli. The recombinant protein is soluble only under reducing conditions, but alkylation with iodoacetamide renders it soluble in non-reducing media |
| 2.5.1.78 | expression of beta60 capsid |
| 2.5.1.78 | expression of beta60 capsid carying lumazine synthase activity |
| 2.5.1.78 | gene bls, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant expression of enzyme BLS fused to protein omp31 as His-tagged protein in Escherichia coli strain BL21(DE3) |
| 2.5.1.78 | gene LcLS1, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative real-time PCR expression analysis |
| 2.5.1.78 | gene LcLS2, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative real-time PCR expression analysis |
| 2.5.1.78 | gene RIB4A, recombinant expression of His-tagged enzyme in Escherichia coli |
