EC Number |
---|
2.4.1.80 | - |
2.4.1.80 | cloning and transient expression in GM-95 cell line, mutant cell line containing no enzyme activity, derived from melanoma mutant cell line B16, DNA sequence determination and analysis |
2.4.1.80 | cloning from CHO cell line K1, overexpression in GM3-mutant CHO cell line Z65, DNA and amino acid sequence determination |
2.4.1.80 | cloning from rat brain genetic library and expression in Escherichia coli BL21(DE3) |
2.4.1.80 | cloning of wild-type and construction of knock-out mutant, functional expression in Saccharomyces cerevisiae strain 334, expression of wild-type leads to accumulation of beta-D-glucopyranosyl ceramides, absent in wild-type Saccharomyces cerevisiae, knock-out mutation is not lethal |
2.4.1.80 | expressed as recombinant protein |
2.4.1.80 | expressed in different drug-resistant human cancer cell lines |
2.4.1.80 | expressed in GM95 cells deficient in glycosphingolipids |
2.4.1.80 | expressed in PC12 cells |
2.4.1.80 | expression in an enzyme-deficient strain of Pichia pastoris |