EC Number |
---|
2.3.1.97 | - |
2.3.1.97 | cloning of cardiac muscle enzyme, expression in Escherichia coli, DNA and amino acid sequence determination |
2.3.1.97 | cloning of cDNA from liver mRNA, construction of 2 expression plasmids with ATG at position 28 and 211, respectively, termed h28NMT and h211NMT, functional expression in Escherichia coli and h28NMT in HEK 293 cells |
2.3.1.97 | codon-optimized Nef plasmid and the human NMT plasmid were cotransformed in BL21(DE3) |
2.3.1.97 | construction of expression plasmid encoding the enzyme, lacking the first 8 amino acid residues, fused to a enterokinase cleavage site and a polyhistidine tag, expression in Escherichia coli DH5alpha, cDNA from spleen |
2.3.1.97 | construction of expression plasmid encoding the enzyme, lacking the first 9 amino acid residues, fused to a enterokinase cleavage site and a polyhistidine tag, overexpression in Escherichia coli DH5alpha |
2.3.1.97 | DNA and amino acid sequence determination and analysis, overexpression of the His-tagged NMT in Escherichia coli strain BL21(DE3) pRareS |
2.3.1.97 | expression as His-tagged protein in Escherichia coli |
2.3.1.97 | expression in COS-7 cells |
2.3.1.97 | expression in Escherichia coli |