EC Number   |
|---|
    1.7.3.3 | - |
| 1.7.3.3 | a plasmid containing the AgUOX gene is introduced into the expression host cell Escherichia coli DH1 |
| 1.7.3.3 | complete cDNA and genomic DNA fragment coding for urate oxidase is isolated and characterized, heterologous expression in Escherichia coli |
| 1.7.3.3 | developing of a method for genetically incorporating p-azido-L-phenylalanine into target protein in Escherichia coli in a site-specific manner utilizing a tyrosyl suppressor tRNA/aminoacyl-tRNA synthetase system, substitution of p-azido-L-phenylalanine for F170 or F281 in urate oxidase, optimization of the system by adding a Shine-Dalgarno sequence and tandem suppressor tRNA in order to increase the expression levels of tyrosyl suppressor tRNA and aminoacyl-tRNA synthetase |
| 1.7.3.3 | expressed in Chinese hamster ovary cells |
| 1.7.3.3 | expressed in Escherichia coli |
| 1.7.3.3 | expressed in Escherichia coli BL21 (DE3) |
| 1.7.3.3 | expressed in Escherichia coli BL21 (DE3) cells |
| 1.7.3.3 | expressed in Escherichia coli BL21 cells |
| 1.7.3.3 | expressed in Escherichia coli BL21(DE3) cells |
