EC Number |
---|
1.4.1.21 | - |
1.4.1.21 | AspDH gene cluster, gene AspDH expression in Escherichia coli strain BL21(DE3), quantitative real-time PCR expression analysis |
1.4.1.21 | DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli |
1.4.1.21 | expressed in Escherichia coli |
1.4.1.21 | expression in Escherichia coli |
1.4.1.21 | expression of N-terminally His-tagged and GFP-tagged enzyme, using the flexible GGSGG linker, in Escherichia coli. The recombinant tagged aspartate dehydrogenase functions as the biorecognition element, and aspartate-induced conformational change is converted to a fluorescence signal by GFP, method, overview |
1.4.1.21 | gene KPN_03362, DNA and amino acid sequence determination and analysis |
1.4.1.21 | gene nadX, phylogenetic analysis |
1.4.1.21 | gene nadX, the gene forms an operon with the NAD biosynthesis genes nadA and nadC |
1.4.1.21 | ligated into the expression vector pET11a, expression in Escherichia coli strain BL21(DE3) |