EC Number |
---|
1.2.1.28 | - |
1.2.1.28 | expression in Escherichia coli |
1.2.1.28 | expression in Escherichia coli DH10B |
1.2.1.28 | gene mdlD, recombinant epression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
1.2.1.28 | gene xylC, recombinant expression in Escherichia coli strain BL21(DE3) in inclusion bodies. Using higher concentration of lysozyme in lysis buffer, high speed, and long-term centrifugation reduces the protein aggregation |
1.2.1.28 | into the vector pET-28a+ for expression in Escherichia coli BL21 Rosetta cells, into the pEF1-LIS binary vector for transformation of petunia flowers with Agrobacterium tumefaciens, for subcellular localization experiments in Arabidopsis protoplasts GFP fusion proteins are constructed using the vector p326-SGFP |