EC Number |
---|
1.14.19.1 | - |
1.14.19.1 | cDNA fragment corresponding to bp 278-2005 of the sequence was subcloned into a pcDNA3 vector in antisense orientation and stably transfected into SV40-WI38 lung fibroblasts cells, enzyme activity reduced in these stable cell clones, reduced protein level, reduced enzyme expression slows the rate of cell proliferation and suppresses anchorage-independent proliferation, SCD deficiency promotes apoptosis in a ceramide-independent manner |
1.14.19.1 | coexpression of acyl carrier protein isoform I gene and holo-acyl carrier protein synthase of Escherichia coli |
1.14.19.1 | expressed in Escherichia coli Rosetta (DE3) cells |
1.14.19.1 | expressed in HEK-293 cell |
1.14.19.1 | expressed in HEK-293 cells |
1.14.19.1 | expressed in HEK-293A cells |
1.14.19.1 | expressed in HeLa cells, tagged with myc epitope at the C terminus or HA epitope |
1.14.19.1 | expressed in L6GLUT4myc myoblasts, localized in endoplasmic reticulum-like subcellular structures, laser scanning microscopy |
1.14.19.1 | expressed in primary human skeletal muscle cells from obese and lean donors, SCD1 mRNA levels are increased 2.8fold, protein levels are increased 5.7fold in myotubes from obese compared to lean subjects |