EC Number   |
|---|
   1.11.1.5 | - |
| 1.11.1.5 | expressed in Escherichia coli JM109 (DE3) cells |
| 1.11.1.5 | expressed in Escherichia coli strain BL21(DE3) |
| 1.11.1.5 | expression in Escherichia coli |
| 1.11.1.5 | expression in Escherichia coli JM109(DE3) with pETCCP coexpressed with pEC86 |
| 1.11.1.5 | expression of maltose-binding-protein-fusion and tag-free CcP in Escherichia coli strain JM 109, the presence of the MBP tag affects the availability of certain binding sites, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
| 1.11.1.5 | expression of V197C/C128A mutant in Escherichia coli |
| 1.11.1.5 | expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
| 1.11.1.5 | functional expression of deuterated and soluble His-tagged Ccp in Escherichia coli strain BL21(DE3). Introduction of a His-tag at either protein terminus dramatically increases its solubility, allowing preparation of concentrated, stable CcP samples. The engineered His tags neither perturb the structure of the enzyme nor alter the heme environment or its reactivity toward known ligands |
| 1.11.1.5 | gene ccpA, expression of His-tagged CcpA in Escherichia coli strain AS457 |
