EC Number |
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1.1.3.9 | computational active-site models for galactose oxidase for the distinctive oxidation states (Cu2+ protein complex/radical), additional models including solvent molecules and second coordination sphere residues |
1.1.3.9 | expressed in Escherichia coli BL21 cells |
1.1.3.9 | expression in Aspergillus nidulans |
1.1.3.9 | expression in Aspergillus oryzae, Fusarium venenatum |
1.1.3.9 | expression in Escherichia coli |
1.1.3.9 | expression in Pichia pastoris |
1.1.3.9 | functional expression of wild-type and mutant enzymes in Escherichia coli strain BL21 Star (DE3) cytoplasm, best in strain BL21 Star (DE3) at 25°C, induction for 4-6 h is optimal |
1.1.3.9 | gene gao, recombinant expression in in Aspergillus nidulans, Pichia pastoris, and Escherichia coli |
1.1.3.9 | gene gao, recombinant expression of wild-type enzyme and chimeric enzyme mutants GaO-CBM29 and CBM29-GaO in Pichia pastoris strain KM71H |
1.1.3.9 | gene gaoA, DNA and amino acid sequence determination and analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) |