EC Number |
---|
1.1.1.267 | - |
1.1.1.267 | DNA and amino acid sequence determination and analysis, phylogenetic tree, functional complementation of Escherichia coli DXR-knockout mutants |
1.1.1.267 | DNA and amino acid sequence determination and analysis, quantitative expression analysis, expression of the C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) periplasmic space |
1.1.1.267 | DNA and amino acid sequence determination and analysis, semi-quantitative RT-PCR expression pattern analysis, functional recombinant expression under the control of CaMV 35S promoter in Nicotiana tabacum cv. Zhongyan 90 via Agrobacterium-mediated transformation Overexpression of AvDXR in tobacco increases DXRactivity, photosynthetic pigment content and volatile isoprenoid components |
1.1.1.267 | dxr gene amplified and inserted into the pQE9 expression vector resulting in a protein with an amino-terminal His6-tag |
1.1.1.267 | expression in Escherichia coli |
1.1.1.267 | expression in Nicotiana tabacum |
1.1.1.267 | expression in tobacco as green fluorescent protein fusion protein |
1.1.1.267 | expression of an N-terminal His6-tagged enzyme variant comprising the first 389 of 413 residues |
1.1.1.267 | expression of His-tagged wild-type and mutant enzymes in Escherichia coli |