EC Number   |
|---|
    1.1.1.25 | - |
| 1.1.1.25 | DNA and amino acid sequence determination and analysis |
| 1.1.1.25 | DNA and amino acid sequence determination and analysis, expression of Hs6-tagged isozyme 1 in Escherichia coli, transfection of Nicotina plants |
| 1.1.1.25 | expression analysis, phylogenetic analysis |
| 1.1.1.25 | expression in Escherichia coli |
| 1.1.1.25 | expression in Escherichia coli as recombinant N-terminal GST-tagged protein |
| 1.1.1.25 | expression in Escherichia coli in native and His-tagged form |
| 1.1.1.25 | expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
| 1.1.1.25 | expression of wild-type and selenomethionine-labeled enzyme in Escherichia coli strain DL41 |
| 1.1.1.25 | five putative SDH-encoding genes amplified and cloned into either a modified pET28a vector or the p15TVLIC vector, a ligation-independent vector due to incompatible cloning sites between the insert and pET28.3. Expressed in either the Escherichia coli strain BL21 gold (p15TV-LIC constructs) or the Escherichia coli BL21CodonPlus (pET28.3constructs) |
