EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
6.3.5.11 | 2 ATP + (aq)2cobyrinate + 2 L-glutamine + 2 H2O |
76% of the initial velocity with cobyrinate |
Pseudomonas denitrificans (nom. rej.) |
2 ADP + 2 phosphate + (aq)2cobyrinate a,c-diamide + 2 L-glutamate |
- |
? |
6.3.5.11 | 2 ATP + (CN,aq)cobyrinate + 2 L-glutamine + 2 H2O |
- |
Pseudomonas denitrificans (nom. rej.) |
2 ADP + 2 phosphate + (CN,aq)cobyrinate a,c-diamide + 2 L-glutamate |
- |
? |
6.3.5.11 | 2 ATP + cobyrinate + 2 ammonia + 2 H2O |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
2 ADP + 2 phosphate + cobyrinate a,c-diamide |
- |
? |
6.3.5.11 | 2 ATP + cobyrinate + 2 ammonia + 2 H2O |
- |
Pseudomonas denitrificans (nom. rej.) |
2 ADP + 2 phosphate + cobyrinate a,c-diamide |
- |
? |
6.3.5.11 | 2 ATP + cobyrinate + 2 L-glutamine + 2 H2O |
- |
Pseudomonas denitrificans (nom. rej.) |
2 ADP + 2 phosphate + cobyrinate a,c-diamide + 2 L-glutamate |
- |
? |
6.3.5.11 | 2 ATP + cobyrinate + 2 L-glutamine + 2 H2O |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
2 ADP + 2 phosphate + cobyrinate a,c-diamide + 2 L-glutamate |
the hydrolysis of glutamine and the synthesis of the cobyrinic acid a,c-diamide product are uncoupled. CbiA catalyzes the sequential amidation of the c- and a-carboxylate groups of cobyrinic acid via the formation of a phosphorylated intermediate. CbiA catalyzes the amidation of the c-carboxylate, and then the intermediate is released into solution and binds to the same catalytic site for the amidation of the a-carboxylate |
? |
6.3.5.11 | 2 ATP + cobyrinate g-monoamide + L-glutamine + 2 H2O |
145% of the initial velocity with cobyrinate |
Pseudomonas denitrificans (nom. rej.) |
2 ADP + phosphate + cobyrinate c,g-diamide + L-glutamate |
product is 83% cobyrinate c,g-diamide, 17% cobyrinate a,c,g-triamide |
? |
6.3.5.11 | 2 ATP + hydrogenobyrinate + 2 L-glutamine + 2 H2O |
- |
Pseudomonas denitrificans (nom. rej.) |
2 ADP + 2 phosphate + hydrogenobyrinate a,c-diamide + 2 L-glutamate |
- |
? |
6.3.5.11 | ATP + (CN,aq)cobyrinate c-monoamide + L-glutamine + H2O |
- |
Pseudomonas denitrificans (nom. rej.) |
ADP + phosphate + (CN,aq)cobyrinate a,c-diamide + L-glutamate |
- |
? |
6.3.5.11 | ATP + cobyrinate a-monoamide + L-glutamine + H2O |
81% of the initial velocity with cobyrinate |
Pseudomonas denitrificans (nom. rej.) |
ADP + phosphate + cobyrinate a,c-diamide + L-glutamate |
product is 100% cobyrinate a,c-diamide |
? |