EC Number |
Storage Stability |
---|
4.1.1.32 | -15°C, mitochondrial enzyme, stable for several months |
4.1.1.32 | -20°C, 0.2 M phosphate, DTT and glycerol, stable for 1 month |
4.1.1.32 | -70°C, 0.05 M potassium phosphate, pH 6.8, 0.2 M sucrose, stable for at least 21 months |
4.1.1.32 | -80°C, more than 1 year, the enzyme remains stable |
4.1.1.32 | 4°C, 3 weeks, stable |
4.1.1.32 | 4°C, Co3+-PEPCK with Co3+ bound to enzyme at site n1, 1 week, stable |
4.1.1.32 | 4°C, Cr3+-PEPCK with Cr3+ bound to enzyme at site n1, at least 3 days, stable |
4.1.1.32 | 4°C, in absence of metal ions, under N2, protein concentration 0.5 mg/ml or higher, stable for at least 14 days |
4.1.1.32 | 4°C, purified recombinant enzyme, 100 mM sodium phosphate buffer, pH 7, 100 mM NaCl, 1 month, 24% loss of activity |
4.1.1.32 | 4°C, purified recombinant His-tagged enzyme, 100 mM sodium phosphate buffer, pH 7, 100 mM NaCl, 2 months, 25% loss of activity |