EC Number   |
Storage Stability |
|---|
   3.1.1.29 | -10°C, 50% glycerol, purified enzyme is stable for several months |
| 3.1.1.29 | -10°C, in presence of 1 mg/ml bovine serum albumin, no loss of activity |
| 3.1.1.29 | -20°C, buffer solution: 0.05 M Tris-HCl, pH 7.0, 0.01 M 2-mercaptoethanol, 0.01 M magnesium acetate, or as an ammonium sulfate precipitate, 2 months without significant loss of activity |
| 3.1.1.29 | 22°C, 20 mM Bis-Tris pH 6.6, 50 mM NaCl, 2 mM dithiothreitol, several weeks, without loss of activity or precipitation |
| 3.1.1.29 | 22°C, 20 mM sodium acetate, 150 mM sodium chloride, 2 mM dithiothreitol, pH 5.0, several months, minimal loss of activity |
| 3.1.1.29 | PTRHD1 storage buffer is optimized in two stages using hanging microdrop screening, overview. The most favorable buffer for long-term storage and stability of PTRHD1 is 20 mM MES, 150 mM sodium chloride, pH 6.5. This buffer extends the soluble half-life at 4°C from days to many months for PTRHD1 concentrated to 0.5 mM or greater. The addition of other stabilizers (i.e. glycerol, PEG, etc.) is not pursued due to the tremendous increase in ability to store soluble PTRHD1 in solution |
