EC Number |
Storage Stability |
---|
2.4.2.8 | -20°C, dilute aqueous purified enzyme preparation, a few weeks |
2.4.2.8 | -20°C, purified enzyme, 6 mM 5-phospho-alpha-D-ribose 1-diphosphate, 2 mM MgCl2, stable for at least a month |
2.4.2.8 | -20°C, several months |
2.4.2.8 | -70°C, wild-type enzyme and mutants, except mutant S95C, stable up to 6 months |
2.4.2.8 | -80°C |
2.4.2.8 | -80°C, mutant and wild-type enzyme, stable up to 3 years |
2.4.2.8 | 0°C, 10 mM phosphate, pH 7.1, 1 mM DTT, 10 mM Mg2+, 2 h, no loss of activity |
2.4.2.8 | 4°C, 50 mM Tris-HCl, pH 7.8, 1 M KCl, 10 mM MgCl2, stable at least 1 month |
2.4.2.8 | 4°C, 50 mM Tris-HCl, pH 7.8, complete loss of activity after 4 days |
2.4.2.8 | 4°C, freshly purified enzyme, 10 mM phosphate, pH 6.8, 10 mM DTT, 1 mM 5-phosphoribosyl 1-diphosphate, rapid loss of 90% activity within 48 h |