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EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 6.5.1.2-999 - - 1971, 1976, 1977
Display the word mapDisplay the reaction diagram Show all sequences 6.5.1.2-999 - electrochemical assay for DNA ligase activity. The assay exploits the properties of DNA hairpins tethered at one terminus to a gold electrode and labelled at the other with a ferrocene group for rapid characterisation of DNA status by cyclic voltammetry. Successful ligation of nicked DNA hairpins is indicated by retention of the ferrocene couple when exposure to DNA ligase is followed by conditions that denature the hairpin 660708
Display the word mapDisplay the reaction diagram Show all sequences 6.5.1.2-999 - non-isotopic method for the determination of activity of the thermostable NAD-dependent DNA ligase from Thermus thermophilus HB8. The assay uses the cohesive ends of restriction endonuclease digested lambda-DNA as substrate. Separation of ligated from unligated cohesive end-containing fragments via agarose gel electrophoresis, followed by digital image analysis employing a normalization procedure to correct for artifacts of gel electrophoresis, allows the accurate determination of unit ligase activity 662744
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