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EC Number Renatured (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.80- 734243
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.80dialysis against a 10fold volume of 50 mM Tris-HCl pH 8.5, 100 mM NaCl, 10 mM CaCl2, 10 mM zinc acetate, and 2 M urea 733033
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.80recombinant enzymes from Escherichia coli inclusion bodies by three steps of dialysis in buffer 1 containing 1.5 M urea, 50 mM Tris, pH 8, 150 mM NaCl, 5 mM CaCl2, 0.1 mM ZnCl2, 5 mM 2-mercaptoethanol, 1 mM 2-hydroxyethyl disulphide, 0.1% Brij 35 v/v, and 1 mM PMSF, or in buffer 2 containing 50 mM Tris, pH 8, 150 mM NaCl, 5 mM CaCl2, 0.05 mM ZnCl2, 5 mM 2-mercaptoethanol, 1 mM 2-hydroxyethyl disulphide, 0.1% Brij 35 v/v, and 1 mM PMSF, or in buffer 3 containing 50 mM Tris, pH 8.0, 150 mM NaCl, 5 mM CaCl2, 0.05 mM ZnCl2, 0.1% Brij 35 v/v, and 1 mM PMSF 755435
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.80refolding of the purified polypeptide to active enzyme by gradient dialysis with urea gradient from 6 M decreasing to 0 M and 2-mercaptoethanol gradient from 150 mM decreasing to 0 mM in the presence of CaCl2 and ZnCl2 638802
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