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EC Number	Renatured (Commentary)	Reference
3.1.1.73	after treatment above the thermal denaturation temperature followed by cooling to room temperature, FaeI demonstrates spontaneous refolding into an active state	737514
3.1.1.73	the thermal unfolding of the parental enzyme is irreversible on all the tested conditions, while that of the Cys235 mutants is reversible, and their ability to refold is highly dependent on the denaturing temperature. Mutants denatured at 75°C are able to efficiently reverse the unfolding to regain native structure during the cooling process	737333

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Release 2023.1 (January 2023)