EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
   3.1.3.16 | 0.001 |
- |
4-nitrophenyl phosphate |
mutant enzyme D136Y, with 0.05 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37°C |
730314 |
| 3.1.3.16 | 0.0015 |
- |
4-nitrophenyl phosphate |
mutant enzyme S120R, with 0.05 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37°C |
730314 |
| 3.1.3.16 | 0.0115 |
- |
4-nitrophenyl phosphate |
mutant enzyme T101R, with 2 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37°C |
730314 |
| 3.1.3.16 | 0.03 |
- |
RGRGSpSVGGGS |
at pH 7.5 and, temperature not specified in the publication |
748177 |
| 3.1.3.16 | 0.102 |
- |
6,8-difluoro-4-methylumbelliferyl phosphate |
pH 5.0, 20°C, mutant R124E |
751975 |
| 3.1.3.16 | 0.14 |
- |
6,8-difluoro-4-methylumbelliferyl phosphate |
pH 5.0, 20°C, mutant D63N/E132A |
751975 |
| 3.1.3.16 | 0.17 |
- |
ALSAGSNEYLR |
at pH 7.5 and, temperature not specified in the publication |
748177 |
| 3.1.3.16 | 0.229 |
- |
6,8-difluoro-4-methylumbelliferyl phosphate |
pH 5.0, 20°C, mutant E132L |
751975 |
| 3.1.3.16 | 0.28 |
- |
4-nitrophenyl phosphate |
wild type enzyme, with 0.05 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37°C |
730314 |
| 3.1.3.16 | 0.281 |
- |
6,8-difluoro-4-methylumbelliferyl phosphate |
pH 5.0, 20°C, mutant Q136A |
751975 |
