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EC Number	General Information	Commentary	Reference
6.5.1.2	physiological function	DNA ligase enhances efficiency of DNA polymerase in long PCR amplification	707538
6.5.1.2	physiological function	the enzyme repairs 3'-OH/5'-PO4 nicks in duplex DNA via reaction with NAD+ to form a covalent enzyme-(lysyl-Nzeta)-AMP intermediate, transfer of AMP to the nick 5'-PO4 to form an AppDNA intermediate (step 2), and attack of the nick 3'-OH on AppDNA to form a 3'-5' phosphodiester	748783

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Release 2023.1 (January 2023)