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EC Number General Information Commentary Reference
Display the reaction diagram Show all sequences 2.9.1.3evolution the putative bipartite ortholog of the bacterial YbbB gene is identified in twelve additional archaeal genomes. Its function is confirmed as a tRNA 2-selenouridine synthase through biochemical and genetic experiments. A phylogenetic analysis of bacterial and archaeal versions of this enzyme provides insights into the early evolution of tRNA 2-selenouridine synthase. The active site loop containing the YbbB-characteristic CXRGGXRS motif near the C-terminus of the rhodanese domain and the Walker A motif (GX4GKT) at the N-terminus of the P-loop domain are highly conserved among the archaeal and bacterial YbbB sequences. The identified archaeal YbbB orthologs represent a bipartite version of bacterial tRNA 2-selenouridine synthase 756718
Display the reaction diagram Show all sequences 2.9.1.3malfunction an Escherichia coli mutant deficient in ybbB is incapable of incorporating selenium into tRNA -, 757134
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