EC Number |
Reaction |
Reference |
---|
1.1.1.141 | (5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ = (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ |
displacement reaction mechanism, at pH 7.0 a kinetically significant ternary complex is formed, while at pH 9.0 the ternary complex is not kinetically significant, Theorell-Chance mechanism |
285916 |
1.1.1.141 | (5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ = (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ |
NAD+ is added first, followed by prostaglandin E2, 15-ketoprostaglandin is released followed by NADH |
285924 |
1.1.1.141 | (5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ = (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ |
ordered bi-bi-mechanism |
285920, 285924 |
1.1.1.141 | (5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ = (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ |
residues and domains involved in catalysis and substrate binding, overview |
668359 |
1.1.1.141 | (5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ = (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ |
single displacement mechanism |
285916, 285923 |
1.1.1.141 | (5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ = (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ |
substrate binidng and catalytic mechanism of the enzyme, homology modeling, docking and molecular dynamics simulation, residues Gln148, Ser138, Tyr151, and Lys155 are important, overview |
667924 |