EC Number   |
|---|
   7.4.2.8 | - |
| 7.4.2.8 | by affinity chromatography, using Talon, Co2+, resin and glutathione-Sepharose beads, and by gel filtration |
| 7.4.2.8 | chitin column chromatography, Q-Sepharose column chromatography, and Superdex 16/600 gel filtration |
| 7.4.2.8 | chitin resin column chromatography, Q Sepharose column chromatography, and Superdex 200 gel filtration |
| 7.4.2.8 | EpsE is copurified with the cytoplasmic domain of EpsL to test whether the ATPase activity of EpsE can be modulated by EpsL, complexes are purified using metal affinity chromatography and gel filtration, monomeric EpsE is obtained following thrombin cleavage of a GST-EpsE fusion protein |
| 7.4.2.8 | glutathione Sepharose column chromatography and Ni-agarose column chromatography |
| 7.4.2.8 | His-tagged EscN is purified from the soluble fraction using nickel-chelating Sepharose, the tag is cleaved with thrombin, cleaved product is purified by Mono-Q anion exchange |
| 7.4.2.8 | His-Trap column chromatography and Superdex 75 gel filtration |
| 7.4.2.8 | N-terminal cytoplasmic domain, with His-tag |
| 7.4.2.8 | Ni-NTA column chromatography |
