EC Number   |
|---|
    6.2.1.1 | - |
| 6.2.1.1 | AF-ACS2 |
| 6.2.1.1 | HisTrap affinity column chromatography |
| 6.2.1.1 | Ni-NTA agarose column chromatography |
| 6.2.1.1 | Ni-NTA column chromatography |
| 6.2.1.1 | Ni-NTA resin column chromatography, and Superdex 200 gel filtration |
| 6.2.1.1 | nickel ion affinity chromatography |
| 6.2.1.1 | partial |
| 6.2.1.1 | recombinant alpha and beta subunits from Escherichia coli strain BL21(DE3) by heat tteatment at 90°C for 30 min, hydrophobic interaction chromatography, and gel filtration |
| 6.2.1.1 | recombinant C-terminally His-tagged ACS from Escherichia coli strain BL21(DE3) by nickel affinity chromatography under anaerobic conditions |
