EC Number |
---|
4.3.3.7 | - |
4.3.3.7 | by anion-exchange chromatography, hydrophobic interaction and gel filtration |
4.3.3.7 | by centrifugation and gel filtration |
4.3.3.7 | by centrifugation and gel filtration, to homogeneity |
4.3.3.7 | by centrifugation, anion-exchange liquid chromatography and gel filtration, 2fold, more than 95% pure |
4.3.3.7 | by gel filtration and ultrafiltration |
4.3.3.7 | by gel filtration, recombinant enzyme in the absence of the substrate pyruvate (with a yield of 36.3%) and presence of the substrate pyruvate (with a yield of 98%) |
4.3.3.7 | by sonication, anion-exchange and hydrophobic interaction liquid chromatography |
4.3.3.7 | enzyme expressed from plasmid in strain XL1-Blue, 69fold |
4.3.3.7 | gel filtration |