EC Number |
---|
3.8.1.5 | - |
3.8.1.5 | five mini proteins are designed via computer modeling and analysis that mimick the main function of haloalkane dehalogenase. A mini protein with 86 amino acids is chosen for His-tag purification |
3.8.1.5 | gene dspA, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity |
3.8.1.5 | GJ10 |
3.8.1.5 | GJ10 mutant H289Q |
3.8.1.5 | HiTrap Ni-chelating affinity resin chromatography and Superdex 75 gel filtration |
3.8.1.5 | metallo-affinity chromatography |
3.8.1.5 | Ni-NTA agarose column chromatography and Superdex 75 gel filtration |
3.8.1.5 | Ni-NTA agarose column chromatography, UNO Q6 anion exchange column chromatography and Superdex 75 gel filtration |
3.8.1.5 | Ni-NTA column chromatography |