EC Number   |
|---|
    3.5.4.4 | - |
| 3.5.4.4 | gel filtration, ion exchange chromatography, adenosine deaminase forms a complex with dipeptidyl peptidase II, during purification, it is noted that dipeptidyl peptidase II (DPPII; EC 3.4.14.2) fractions possesses adenosine deaminase activity at all steps |
| 3.5.4.4 | homogeneity, protamine sulfate treatment, Q Sepharose chromatography, purine riboside-Sepharose chromatography |
| 3.5.4.4 | isozyme ADA2 400fold from plasma by ammonium sulfate fractionation, anion exchange chromatography, adsorption chromatography, and gel filtration |
| 3.5.4.4 | isozyme ADA2, 138.3fold by immunoglobulin and heparin affinity chromatography in two alternated sequences, and gel filtration |
| 3.5.4.4 | native ADAI 12.3fold by acetone precipitation and ion exchange chromatography, native ADAII 176.6fold by acetone precipitation, ion exchange chromatography, and gel filtration to homogeneity |
| 3.5.4.4 | native enzymes ADAI, ADAII and ADAIII, separation by acetone precipitation and anion exchange chromatography, 2fold purification of ADA1 and 5fold of ADAIII, further purification 62.9fold to homogeneity of ADAII by gel filtration |
| 3.5.4.4 | Ni-NTA column chromatography |
| 3.5.4.4 | purification of the soluble large isozyme complexed with dipeptidyl peptidase IV from blood serum and pleural fluid by anion exchange chromatography, gel filtration, and affinity chromatography steps to about 90% purity |
| 3.5.4.4 | recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography |
